IL-13 induced asthma model in human precision-cut lung slices

نویسندگان

  • O. Danov
  • S. M. Jiménez
  • S. Huang
چکیده

O. Danov1, S. M. Jiménez Delgado1, H. Drake1, S. Seehase2, A. M. Hohlbaum3, C. Bersch4, D. Jonigk5, G. Warnecke6, A. Braun1 and K. Sewald1 1Airway Immunology, Fraunhofer Institute for toxicology and experimental Medicine, Biomedical Research in endstage and Obstructive lung Disease Hannover (BReAtH), Member of the German Centre for lung Research (DZl), Hannover, Germany; 2Clinical & experimental Pathology, Research Center Borstel, leibniz Center for Medicine and Biosciences, Airway Research Center North (ARCN), Member of the German Center for lung Research, Borstel, Germany; 3R&D, Pieris AG, FreisingWeihenstephan, Germany; 4Institute for Pathology, KRHKlinikum Nordstadt, Hannover, Germany; 5Institute for Pathology, Hannover Medical School, Hannover, Germany; 6Cardiothoracic, transplantation and Vascular Surgery (HttG), Hannover Medical School, Hannover, Germany [email protected]

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Airway Smooth Muscle Sensitivity to Methacholine in Precision-Cut Lung Slices (PCLS) from Ovalbumin-induced Asthmatic Mice

Asthma is a chronic inflammatory disease characterized by airway hyperresponsiveness (AHR) and reversible airway obstruction. Methacholine (MCh) is widely used in broncho-provocation test to evaluate airway resistance. For experimental investigation, ovalbumin-induced sensitization is frequently used in rodents (Ova-asthma). However, albeit the inflammatory histology and AHR in vivo, it remains...

متن کامل

Targeting phosphoinositide 3-kinase γ in airway smooth muscle cells to suppress interleukin-13-induced mouse airway hyperresponsiveness.

We recently reported that phosphoinositide 3-kinase γ (PI3Kγ) directly regulates airway smooth muscle (ASM) contraction by modulating Ca(2+) oscillations. Because ASM contraction plays a critical role in airway hyperresponsiveness (AHR) of asthma, the aim of the present study was to determine whether targeting PI3Kγ in ASM cells could suppress AHR in vitro and in vivo. Intranasal administration...

متن کامل

Protective effects of phenolic acids on mercury-induced DNA damage in precision-cut kidney slices

Objective(s): Precision-cut tissue slices are considered an organotypic 3D model widely used in biomedical research. The comet assay is an important screening test for early genotoxicity risk assessment that is mainly applied on in vitro models.  The aim of the present study was to provide a 3D organ system for determination of genotoxicity using a modified method of the comet assay since the s...

متن کامل

CB2 Agonist (AM1241) Improving Effect on Ovalbumin-Induced Asthma in Rats

Asthma is a disease characterized by spontaneous contraction of the airways in response to a wide variety of endogenous and exogenous stimuli. Many asthma models are used to mimic the human asthma model in the literature. In order to better understand the role of the cannabinoid (CB) 2 receptor in the ovalbumin (OVA)-induced asthma model, a combination of both selective CB2 agonist (AM1241) and...

متن کامل

CB2 Agonist (AM1241) Improving Effect on Ovalbumin-Induced Asthma in Rats

Asthma is a disease characterized by spontaneous contraction of the airways in response to a wide variety of endogenous and exogenous stimuli. Many asthma models are used to mimic the human asthma model in the literature. In order to better understand the role of the cannabinoid (CB) 2 receptor in the ovalbumin (OVA)-induced asthma model, a combination of both selective CB2 agonist (AM1241) and...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2014